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酶類
編號(hào) |
產(chǎn)品名稱 |
英文名稱 |
規(guī)格 |
CAS號(hào) |
包裝 |
YS-1298 |
熒光素酶 |
Luciferase firefly |
BR,98% |
61970-00-1 |
1毫克 |
簡(jiǎn)介:
熒光素酶/熒光素酶(螢火蟲(chóng))/重組型酵素/Luciferase fireflyBR,98%1毫克,Sigma-Aldrich、Amresco、Amersham、Serva、AppliChem等品牌,61970-00-1 保存-20℃
詳細(xì)介紹:
中文名稱: 熒光素酶
其他中文名稱: 熒光素酶(螢火蟲(chóng));重組型酵素
英文名稱: Luciferase firefly
其他英文名稱: Luciferase from Photinus pyralis (firefly)
產(chǎn)品規(guī)格: BR,98%
包裝: 1毫克
CAS號(hào):61970-00-1
級(jí)別:BR
相關(guān)資料:
Assay:≥98% (SDS-PAGE)
Mol wt:mol wt 120 kDa (two non-identical subunits, each containing four free thiol groups, one of which is necessary for activity)
Protein:10-35% E1%/280
Measuring range:≤1 femtomole ATP sensitivity, (using 0.2 μg of luciferase and suitably sensitive liquid scintillation counters or luminometers)
Foreign activity:ATPase ≤5 nanomolar units/mg protein;Nucleoside diphosphokinase ≤20 nanomolar units/mg protein
活力定義:One light unit produces a biometer peak height equivalent to 0.02 μCi of 14C in PPO/POPOP cocktail. Light units measured in 50 μl assay mixture containing 5 pmol ATP and 7.5 nmol luciferin in Tris-glycine buffer, pH 7.6, at 25 °C
產(chǎn)品描述:Two contaminant, ATP-consuming activities are assayed for in this product, ATPase and nucleoside diphosphokinase. These impurities are found to be less than 5 nanomolar units/mg protein and less than 20 nanomolar units/mg protein, respectively.
Note: Prior to 1991, a unit of firefly luciferase activity was defined as that amount which will produce 1.0 nanomole of pyrophosphate per minute at pH 7.7, 25 °C, using a system containing 0.6 mM ATP and 0.1 mM D-luciferin. The former nanomolar unit is equivalent to approximately 1.3 x 106 light units
性狀:粉末。EC:1.13.12.7
用途:生化研究。
保存:-20℃。保質(zhì)期1年